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KMID : 0368420130560040232
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Journal of Plant Biology
2013 Volume.56 No. 4 p.232 ~ p.242
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Molecular genetic characterization of rice seed lipoxygenase 3 and assessment of its effects on seed longevity
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Long Qizhang
Zhang Wenwei
Wang Peng
Shen Wenbiao
Zhou Tong
Liu Nannan
Wang Ren
Jiang Ling
Huang Jiexue
Wang Yihua
Liu Yuqiang
Wan Jianmin
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Abstract
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Lipoxygenases (LOXs) are enzymes involved in lipid peroxidation. Here we reported the identification, molecular and functional characterization of the gene encoding rice (Oryza sativa L.) seed LOX3 (sLOX3). Via a map-based cloning strategy we identified Os03g0700400 as the candidate gene encoding sLOX3. Further functional complementary test and biochemical characterization of the recombinant Os03g0700400 protein verified the identification. The sLOX3 gene was highly expressed in roots, moderately in embryos and very weakly in leaves, leaf sheaths and stems. Transient expression experiment (in rice protoplasts) and subsequent laser confocal microscopic analysis demonstrated that the sLOX3 protein was localized into the cytosol. We next showed that overexpression of sLOX3 in a japonica sLOX3-normal rice cultivar, Wuyunjing 7 accelerated the decrease of seed germination ability when the seeds were routinely stored, which demonstrated that sLOX3 had a negative effect on seed longevity (storability). Meanwhile, an increased occurrence of embryo decay was observed in the same transgenic seeds, suggesting that sLOX3 might negatively affect seed longevity by facilitating colonization of particular seed pathogens. Our result forwarded the understanding of the effects of 9-LOX on rice seed longevity.
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KEYWORD
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Cloning, Lipoxygenase, Prokaryotic expression, Seed longevity, Subcellular localization
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